Permeability of red blood cell membrane to HCO3-

Value 1.64e-3 cm/sec Range: ±0.00015 Table - link cm/sec
Organism Human Homo sapiens
Reference Forster RE, Gros G, Lin L, Ono Y, Wunder M. The effect of 4,4'-diisothiocyanato-stilbene-2,2'-disulfonate on CO2 permeability of the red blood cell membrane. Proc Natl Acad Sci U S A. 1998 Dec 22 95(26):15815-20. p.15818 table 1PubMed ID9861053
Method "The reactions take place in an airtight 3-ml glass water-jacketed and stirred chamber connected to the ion source of a mass spectrometer through a thin 0.012-mm-thick Teflon membrane supported by a sintered glass disc, through which gases are dissolved in the solution diffuse. The chamber has a glass pH electrode and a removable stopper for the addition of reactants."
Comments "DIDS (100µM) did not alter the calculated CA [carbonic anhydrase] activity in the intact red cells, but did lower Pm,HCO3- to about half. The interesting finding is that membrane CO2 permeability, from a value of 1cm/sec or higher in the control experiments, was lowered by the drug to a value of 0.1cm/sec."
Entered by Uri M
ID 110616