mCherry maturation half-time

Range ~40 min
Organism Bacteria Escherichia coli
Reference Khmelinskii A et al., Tandem fluorescent protein timers for in vivo analysis of protein dynamics. Nat Biotechnol. 2012 Jun 24 30(7):708-14. doi: 10.1038/nbt.2281. p.709 left column 3rd paragraph (this paper deals with budding yeast but cited value measured in primary ref within E. coli)PubMed ID22729030
Primary Source Merzlyak EM et al., Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods. 2007 Jul4(7):555-7. (measured in E. coli, see Fig. S2)PubMed ID17572680
Comments "...[investigators] combined the monomeric red fluorescent protein mCherry, which matures with a half-time of ~40 min [primary source], with a monomeric variant of the superfolder green fluorescent protein sfGFP, which becomes fluorescent within minutes of synthesis [ref 18]. A pool of mCherry-sfGFP molecules should be mostly green-fluorescent shortly after synthesis and gradually acquire red fluorescence over time, such that the ratio of red to green fluorescence is a function of the age of the protein pool (Fig. 1b)."
Entered by Uri M
ID 110551