| Range |
~4e-6 misincorporations/site
|
| Organism |
Nematode Caenorhabditis elegans |
| Reference |
Gout JF, Thomas WK, Smith Z, Okamoto K, Lynch M. Large-scale detection of in vivo transcription errors. Proc Natl Acad Sci U S A. 2013 Nov 12 110(46):18584-9. doi: 10.1073/pnas.1309843110. abstract & p.18588 left column 3rd paragraphPubMed ID24167253
|
| Method |
P.18584 right column bottom paragraph: "[Researchers] describe a unique method
for identifying transcription errors by sequencing multiple cDNAs originating from the same mRNA molecule, using a barcoding strategy to trace back the origin of individual cDNAs." P.18588 left column 3rd paragraph: "In this study, [researchers] have described a unique cDNA library preparation technique and the associated bioinformatic analyses that allow for detection of transcription errors in RNA-seq data." |
| Comments |
Abstract: "Here [researchers] report a unique cDNA library preparation technique that allows error detection in natural transcripts at the transcriptome-wide level. Application of this method to the model organism Caenorhabditis elegans revealed a base misincorporation rate in mRNAs of ~4×10^-6 per site, with a very biased molecular spectrum." P.18588 left column 3rd paragraph: "To demonstrate the feasibility of [their] method, [researchers] applied it to the transcriptome of C. elegans and detected dozens of transcription errors, yielding
a base substitution transcription error rate of ~4×10^-6." |
| Entered by |
Uri M |
| ID |
110503 |