Change in volume of guard cells during stomatal movement

Range >40 %
Organism Broadbean Vicia faba
Reference Gao XQ, Li CG, Wei PC, Zhang XY, Chen J, Wang XC. The dynamic changes of tonoplasts in guard cells are important for stomatal movement in Vicia faba. Plant Physiol. 2005 Nov139(3):1207-16. p.1207 right column 2nd paragraphPubMed ID16244153
Primary Source Shope JC, DeWald DB, Mott KA (2003) Changes in surface area of intact guard cells are correlated with membrane internalization. Plant Physiol 133: 1314–1321PubMed ID14551331
Method (primary source abstract:) "A membrane-specific fluorescent dye was used to identify the plasma membrane, and confocal microscopy was used to acquire a series of optical paradermal sections of the guard cell pair at each osmotic potential. Solid digital objects representing the guard cells were created from the membrane outlines identified in these paradermal sections, and surface area, volume, and various linear dimensions were determined for these solid objects...Surface area decreased by as much as 40% when external osmotic potential was increased from 0 to 1.5 MPa, and surface area varied linearly with volume."
Comments "During stomatal movement, the volume of the guard cells can change by more than 40% (primary source)."
Entered by Uri M
ID 110347