| Range |
Table - link Transformants/μgDNA
|
| Organism |
Yeast |
| Reference |
Suga M, Hatakeyama T. High-efficiency electroporation by freezing intact yeast cells with addition of calcium. Curr Genet. 2003 Jun43(3):206-11. p.210 table 2PubMed ID12684838
|
| Method |
"Cells of all
strains were frozen in 0.6M sorbitol with 10mM CaCl2 and
10mM Hepes, pH 7.5. Cell concentrations during the electric pulse
were 1?10^9cells/ml and 2?10^9cells/ml for non-frozen cells and
cells after freezing with calcium, respectively. The electric pulse
settings (for 25µF, 200O) were 10.0kV/cm for Sch. pombe and
11.0kV/cm for Saccharomyces cerevisiae, for both non-frozen cells
and cells after freezing." |
| Comments |
"Data represent averages ±SD for five
independent experiments" |
| Entered by |
Uri M |
| ID |
110141 |