Transcription elongation rate

Range ~40 Table - link nts/sec
Organism Budding yeast Saccharomyces cerevisiae
Reference Kos M, Tollervey D. Yeast pre-rRNA processing and modification occur cotranscriptionally. Mol Cell. 2010 Mar 26 37(6):809-20. doi: 10.1016/j.molcel.2010.02.024. abstract and p.811 left column 4th paragraph and p.816 table 1 & p.817 right column 5th paragraphPubMed ID20347423
Method P.817 right column 3rd paragraph: "[Researchers] report the development of techniques for sampling metabolically labeled yeast cells at 10 s intervals, and the use of the derived quantitative data to populate a mathematical model of the processing pathway."
Comments P.817 right column 5th paragraph: "With this insight,the transcription time of 35S synthesis can be reliably determined—from the time required to reach steady state, less the label equilibration time and the 35S life-time, which [researchers] knew to be very short (~10 s) (Venema and Tollervey, 1999). At 30°C in [researchers'] strain (which is derived from W303), this was found to be 170 s, corresponding to 40 nt/s."
Entered by Uri M
ID 109913