| Comments |
"The in vivo measurement allowed accurate
determination of organelle-specific pH, determining a constant pHcyt of 7.2 and a constant pHmit
of 7.5 in cells exponentially growing on glucose." "[Researchers] determined a pHcyt of 7.2±0.2
and a pHmit of 7.5±0.2 in cells growing on glucose. These
specific pH values appear to be tightly regulated and maintained in a broad range of external pH values, ranging
from pH 3.0 to 7.5 (Figs 2c, 5a–c and 6). Previously
reported pHi values were lower (Bracey et al., 1998
Fernandes et al., 2003 Guldfeldt & Arneborg, 1998). This is
most likely due to the methods used, which determine an
average pH over whole cells including the vacuole. This will
result in significantly lower values." |