Duration of replication cycle of Pandoraviruses in Acanthamoeba castellanii

Range 10 to 15 hours
Organism Viruses Pandoravirus spp.
Reference Philippe N et al., Pandoraviruses: amoeba viruses with genomes up to 2.5 Mb reaching that of parasitic eukaryotes. Science. 2013 Jul 19 341(6143):281-6. doi: 10.1126/science.1239181. p.282 left column 2nd paragraphPubMed ID23869018
Method P.282 left column bottom paragraph: "To distinguish whether the parasites were cellular or viral in nature, [researchers] imaged their propagation in axenic Acanthamoeba cultures over an entire multiplication cycle, starting from purified particles."
Comments P.282 left column bottom paragraph: "The replication cycle of Pandoraviruses in Acanthamoeba castellanii lasts from 10 to 15 hours and is initiated by the internalization of individual particles via phagocytic vacuoles. The particles then empty the content of their internal compartment into the Acanthamoeba cytoplasm through their apical pore."
Entered by Uri M
ID 109555