| Value |
57
Days
|
| Organism |
Mouse Mus musculus |
| Reference |
Cheshier SH, Morrison SJ, Liao X, Weissman IL. In vivo proliferation and cell cycle kinetics of long-term self-renewing hematopoietic stem cells. Proc Natl Acad Sci U S A. 1999 Mar 16 96(6):3120-5. abstract, p.3123 left column 2nd paragraph and p.3125 left column top paragraphPubMed ID10077647
|
| Method |
Study of BrdUrd (Bromodeoxyuridine) incorporation kinetics |
| Comments |
"The potential doubling time of asynchronously dividing cells is the amount of time necessary for 77% of cells to incorporate BrdUrd (ref 25). Thus, the potential doubling time of all MPP [multipotent progenitors] and LT-HSC was 7.7 days and 17.8 days, respectively. Finally, the 99% BrdUrd-incorporation point was extrapolated from the log-linear plot as about 57 days for LT-HSC." "[Researchers'] data demonstrate that LTHSC
enter the cell cycle in an asynchronous manner, that proliferation was not dominated by a few clones, and that on
average 99% of LT-HSC divide every 57 days." |
| Entered by |
Uri M |
| ID |
109232 |