| Value |
50
min
|
| Organism |
Fruit fly Drosophila melanogaster |
| Reference |
Drocco JA, Grimm O, Tank DW, Wieschaus E. Measurement and perturbation of morphogen lifetime: effects on gradient shape. Biophys J. 2011 Oct 19 101(8):1807-15. doi: 10.1016/j.bpj.2011.07.025. p.1813 left column top paragraphPubMed ID22004733
|
| Method |
Abstract: "Using a method that employs repeated photoswitching of a fusion of the morphogen Bicoid (Bcd) and the photoconvertible fluorescent protein Dronpa, [investigators] measure and modify the lifetime of Dronpa-Bcd in living Drosophila embryos." |
| Comments |
p.1813 left column top paragraph: "A similar estimate for general cytoplasmic flux between cycles 12 and 14 was obtained using live imaging to follow the redistribution of the Histone-RFP. Correcting for this flux has a minor effect on the degradation rate as a function of developmental time (Fig. 4 e, red line) but yields a degradation rate before cycle 14 of 0.020±0.006 min^-1, and a lifetime estimate of 50 min, identical to that measured at the beginning of cycle 14. [Researchers] conclude that from cycle 11 to the beginning of cycle 14, the degradation rate of Bcd protein is approximately constant." For whole paragraph from p.1812 right column bottom paragraph to p.1813 left column top paragraph see link |
| Entered by |
Uri M |
| ID |
109197 |