Kcat/Km of mitochondrial complex I for NADH oxidation by the flavin

Range ~1e8 M^-1×sec^-1
Organism Cow Bos Taurus
Reference Hirst J. Mitochondrial complex I. Annu Rev Biochem. 2013 Jun 2 82 :551-75. doi: 10.1146/annurev-biochem-070511-103700. p.557 left column bottom paragraphPubMed ID23527692
Primary Source Birrell JA, Yakovlev G, Hirst J. Reactions of the flavin mononucleotide in complex I: a combined mechanism describes NADH oxidation coupled to the reduction of APAD+, ferricyanide, or molecular oxygen. Biochemistry. 2009 Dec 22 48(50):12005-13. doi: 10.1021/bi901706w.PubMed ID19899808
Comments "For B. taurus complex I, kcat for NADH oxidation by the flavin is >15,000 s^-1, and kcat/KM is ~10^8M^-1 s^-1, approaching the value for diffusion control (primary source)."
Entered by Uri M
ID 109179