| Value |
3640
molecules/vesicle
|
| Organism |
Rat Rattus norvegicus |
| Reference |
Riveros N, Fiedler J, Lagos N, Muñoz C, Orrego F. Glutamate in rat brain cortex synaptic vesicles: influence of the vesicle isolation procedure. Brain Res. 1986 Oct 29 386(1-2):405-8. abstract and p.407 right column bottom paragraphPubMed ID2877717
|
| Method |
(Ref abstract:) "A slightly modified Kadota and Kadota procedure (J. Cell Biol., 58 (1973) 135 PMID 4125368) gave synaptic vesicles of a very high purity that were filtered on a Sepharose 4B column, and there, the synaptic vesicle fraction of highest purity was estimated to contain 3640 glutamate molecules in each glutamatergic vesicle." |
| Comments |
"Therefore, if one assumes that 30% of
all the SVs [synaptic vesicles] present operate with glutamate as a transmitter,
this would raise the number of glutamate molecules/
SV to 3640, that is quite close to the estimated
quantum size of 6000 ACh molecules in cholinergic
motor nerve endings (Kuffler et al., 1975 PMID 171380)." |
| Entered by |
Uri M |
| ID |
108622 |