Concentration of glutamate in synaptic vesicles

Range ~60 mM
Organism Rat Rattus norvegicus
Reference Scimemi A, Beato M. Determining the neurotransmitter concentration profile at active synapses. Mol Neurobiol. 2009 Dec40(3):289-306. p.291 left column top paragraphPubMed ID19844813
Primary Source Burger et al., Synaptic vesicles immunoisolated from rat cerebral cortex contain high levels of glutamate. Neuron. 1989 Dec3(6):715-20.PubMed ID2577130
Method Given that (1) there is ~0.8µmol glutamate/mg synaptophysin, (2) synaptophysin represents ~7% of total vesicle proteins, (3) the volume of synaptic vesicles is assumed to be ~1µl/mg total protein, it was suggested that the glutamate concentration in synaptic vesicles is ~60 mM, and certainly <150 mM, a value imposed by the Donnan law (primary source).
Comments These estimates are in rough agreement with those obtained from synaptic vesicles isolated with other purification protocols, yielding values of 70–210 mM (Riveros et al., 1986 PMID 2877717)
Entered by Uri M
ID 107302