| Range |
Table - link
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Proshkin S, Rahmouni AR, Mironov A, Nudler E. Cooperation between translating ribosomes and RNA polymerase in transcription elongation. Science. 2010 Apr 23 328(5977):504-8. p.506 table 2PubMed ID20413502
|
| Primary Source |
29) Varenne S, Buc J, Lloubes R, Lazdunski C. Translation is a non-uniform process. Effect of tRNA availability on the rate of elongation of nascent polypeptide chains. J Mol Biol. 1984 Dec 15 180(3):549-76. AND (6) Vogel U, Jensen KF. The RNA chain elongation rate in Escherichia coli depends on the growth rate. J Bacteriol. 1994 May176(10):2807-13.PubMed ID6084718, 7514589
|
| Method |
"To calculate the elongation rate, the
Escherichia coli culture was induced with IPTG,
and the time that elapsed between the appearance
of a specific hybridization signal from
probes complementary to the 5' and 3' segments
of the lacZ transcript was determined by dotblot
hybridization (Fig. 1 and fig. S1) (refs 5, 6)." |
| Comments |
"Remarkably, transcription of rplB-tufA matched
its fast translation rate and was more than 1.5
times as fast as that of srb4 (Fig. 1D and Table 2).
Moreover, genes carrying an intermediate number
of rare codons (lacZ and infB) predictably
displayed intermediate rates of transcription
(Table 2). Thus, codon usage is a key determinant
of transcription rate." |
| Entered by |
Uri M |
| ID |
108490 |