| Range |
0.9 to 1.3 mM
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Chen J, Brevet A, Fromant M, Lévêque F, Schmitter JM, Blanquet S, Plateau P. Pyrophosphatase is essential for growth of Escherichia coli. J Bacteriol. 1990 Oct172(10):5686-9. p.5687 right column 3rd paragraphPubMed ID2170325
|
| Method |
Pyrophosphatase activity in the cell extract was measured
in a 0.1-ml assay mixture containing 25 mM Tris hydrochloride
(pH 7.8), 20 mM KCl, 2.5 mM MgCl2, 0.1 mM PPi,
100,000 dpm of 32PP, and appropriately diluted crude extract
solution. |
| Comments |
During growth,
cellular PPi remained nearly constant, between 0.9 and 1.3
mM. It is noteworthy that this concentration is identical to
that in the parental strain K37. |
| Entered by |
Uri M |
| ID |
108173 |