| Range |
7.98±0.07 in HeLa cells: 7.91±0.16 in rat cardiomyocytes: 7.78±0.17 in human ECV304 cells unitless
|
| Organism |
Mammals |
| Reference |
H. S. Haraldsdottir, I. Thiele, & R. M. T. Fleming, Quantitative Assignment of Reaction Directionality in a Multicompartmental Human Metabolic Reconstruction, Biophysical J, February 17, 2012, supporting online material p.16/27 1st paragraphPubMed ID22768925
|
| Primary Source |
9) Llopis J, McCaffery JM, Miyawaki A, Farquhar MG, Tsien RY. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc Natl Acad Sci U S A. 1998 Jun 9 95(12):6803-8. p.6805 right column 2nd paragraph (10) Porcelli AM, Ghelli A, Zanna C, Pinton P, Rizzuto R, Rugolo M. pH difference across the outer mitochondrial membrane measured with a green fluorescent protein mutant. Biochem Biophys Res Commun. 2005 Jan 28 326(4):799-804.PubMed ID9618493, 15607740
|
| Method |
EYFP-mit was targeted to the mitochondrial matrix by fusing it with a short peptide from
a mitochondria specifi c protein. |
| Comments |
Mitochondrial pH was found to be 7.98 ± 0.07 in HeLa cells and 7.91 ± 0.16 in cardiomyocytes (primary source 9). Using the same
method Porcelli et al. measured a pH of 7.78 ± 0.17 in ECV304 cells (human umbilical
vein endothelial cells, primary source 10). |
| Entered by |
Uri M |
| ID |
108124 |