| Range |
~7.2 unitless
|
| Organism |
Rat Rattus norvegicus |
| Reference |
Buckler KJ, Vaughan-Jones RD. Application of a new pH-sensitive fluoroprobe (carboxy-SNARF-1) for intracellular pH measurement in small, isolated cells. Pflugers Arch. 1990 Oct417(2):234-9. p.237 right column bottom paragraphPubMed ID2084617
|
| Method |
P.235 left column 3rd paragraph: "Type-1 cells from the carotid body were isolated from neonatal rats according to the method of Peers (1990)." P.237 right column 2nd paragraph: "Briefly, when one applies either a weak acid or weak base to a cell, the non-ionised form will cross the cell membrane and subsequently release or absorb protons until: (i) the non-ionised form is at an equal concentration on either side of the membrane and (ii) an equilibrium
between charged and uncharged forms is attained within the cell." |
| Comments |
P.237 right column bottom paragraph: "In conclusion, this calibration technique suggested that pHi was about 7.2, which compared favourably with the value of 7.25 determined in the same cell using the nigericin calibration (the results of this latter calibration are indicated on the pHi scale to the left of the recording)." From PMID 22768925: "Buckler and Vaughan-Jones were first to introduce the use of a new pH sensitive
fluorescent probe, carboxy-SNARF-
1 (pKa = 7.5), for measurements of cytosolic pH" |
| Entered by |
Uri M |
| ID |
108096 |