| Range |
~60 mM
|
| Organism |
Rat Rattus norvegicus |
| Reference |
Scimemi A, Beato M. Determining the neurotransmitter concentration profile at active synapses. Mol Neurobiol. 2009 Dec40(3):289-306. p.291 left column top paragraphPubMed ID19844813
|
| Primary Source |
Burger et al., Synaptic vesicles immunoisolated from rat cerebral cortex contain high levels of glutamate. Neuron. 1989 Dec3(6):715-20.PubMed ID2577130
|
| Method |
Given that (1)
there is ~0.8µmol glutamate/mg synaptophysin, (2)
synaptophysin represents ~7% of total vesicle proteins, (3)
the volume of synaptic vesicles is assumed to be ~1µl/mg
total protein, it was suggested that the glutamate concentration
in synaptic vesicles is ~60 mM, and certainly
<150 mM, a value imposed by the Donnan law (primary source). |
| Comments |
These
estimates are in rough agreement with those obtained from
synaptic vesicles isolated with other purification protocols,
yielding values of 70–210 mM (Riveros et al., 1986 PMID 2877717) |
| Entered by |
Uri M |
| ID |
107302 |