Dissociation constant of avidin and biotin
| Value | 1E-15 M |
|---|---|
| Organism | Generic |
| Reference | Green NM. Avidin. 1. The use of (14-C) biotin for kinetic studies and for assay. Biochem J. 1963 Dec89: 585-91 p.591 left column bottom paragraphPubMed ID14101979 |
| Method | A rapid method employing radioactive biotin |
| Comments | The dissociation constant of the complex calculated from the rate constants was about 10^-15M, corresponding to a free energy change of 20 kcal/mole of biotin bound. |
| Entered by | Uri M |
| ID | 107216 |