Folding free energy of the average protein

Range -7.1 E. coli: -8.8 S. cerevisiae: -8:2 C. elegans kcal/mol
Organism Various
Reference Ghosh K, Dill K. Cellular proteomes have broad distributions of protein stability. Biophys J. 2010 Dec 15 99(12):3996-4002. p.3997 left column 1st paragraph under "Results"PubMed ID21156142
Method For E. coli, at 37°C, the average protein has a folding free energy of <?G>=-11.9RT~-7.1kcal/mol, where RT is the gas constant multiplied by absolute temperature (for which researchers used T=300 K here). For yeast (Saccharomyces cerevisiae (SCE)), for which <L>~475 and a=1.56, the average stability is <?G>=-14.7RT~-8.8kcal/mol. For the worm (Caenorhabditis elegans (CEL)), for which <L>~425 and a=1.23, the average protein stability is <?G>=-13.7RT~-8.2kcal/mol. Where <L> is the chain length and in the case of gamma distribution, the protein length variation can be measured by a shape parameter, a. The lower the a value, the greater the variation (Zhang 2000).
Comments The standard deviations of the stability distributions are 4.3 RT, 7.5 RT, and 7.5 RT for E. coli, yeast, and worm, respectively.
Entered by Uri M
ID 107069