Distribution of 100 protein removal rates and half-lives under normal growth
Range | Figure link - link |
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Organism | Human Homo sapiens |
Reference | Eden E, Geva-Zatorsky N, Issaeva I, Cohen A, Dekel E, Danon T, Cohen L, Mayo A, Alon U. Proteome half-life dynamics in living human cells. Science. 2011 Feb 11 331(6018):764-8 p.766 figure 2C,DPubMed ID21233346 |
Method | Researchers present an assay called “bleach-chase” for measuring protein half-lives that does not require translation inhibitors, antibodies, or radioactive labeling. In the bleach-chase method, the fluorophore of the tagged protein is bleached using a brief pulse of light, irreversibly rendering it nonfluorescent. |
Comments | H1299 human non–small cell lung cancer cell line. See note beneath table |
Entered by | Uri M |
ID | 106835 |