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Protein half-lives under normal growth condition as measured by bleach chase in H1299 human non–small cell lung cancer cell

Range	45min to 22.5 hours Table - link Hours
Organism	Human Homo sapiens
Reference	Eden E, Geva-Zatorsky N, Issaeva I, Cohen A, Dekel E, Danon T, Cohen L, Mayo A, Alon U. Proteome half-life dynamics in living human cells. Science. 2011 Feb 11 331(6018):764-8 Supporting online material pp.17-19 table S4PubMed ID21233346
Method	Researchers present an assay called “bleach-chase” for measuring protein half-lives that does not require translation inhibitors, antibodies, or radioactive labeling. YFP fluorophores were bleached by brief (30- to 480-s) pulses of light, resulting in a 10 to 60% decrease in tag fluorescence.
Entered by	Uri M
ID	106814