Cell cycle time of H1299 human non–small cell lung cancer cell

Range 22.5±2.6hours bleached cell: 23.2±2.5hours control cell Hours
Organism Human Homo sapiens
Reference Eden E, Geva-Zatorsky N, Issaeva I, Cohen A, Dekel E, Danon T, Cohen L, Mayo A, Alon U. Proteome half-life dynamics in living human cells. Science. 2011 Feb 11 331(6018):764-8 p.765 left column bottom paragraph and p.766 middle column top paragraphPubMed ID21233346
Method Researchers present an assay called “bleach-chase” for measuring protein half-lives that does not require translation inhibitors, antibodies, or radioactive labeling. YFP fluorophores were bleached by brief (30- to 480-s) pulses of light, resulting in a 10 to 60% decrease in tag fluorescence.
Entered by Uri M
ID 106813