PPi concentration in exponentially growing cells of Escherichia coli K-12

Value 0.5 mM
Organism Bacteria Escherichia coli
Reference Kukko E, Heinonen J. The intracellular concentration of pyrophosphate in the batch culture of Escherichia coli. Eur J Biochem. 1982 Oct127(2):347-9 p.349 left column top paragraphPubMed ID6291941
Method The recovery of PPi at different growth phases was tested by adding 100 pmol labelled [32P]PPi to the cultures immediately before killing
Comments According to researchers' results the level of PPi in the growing cells of Exherichia coli is 2.5 nmol/mg protein, which is equivalent to the intracellular concentration of 0.5 mM, if there are 2.7 ml intracellular water/g dry weight. See BNID 104792
Entered by Uri M
ID 106780