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Spontaneous mutation rates among RNA viruses: genome size, mutations per bp per replication and mutations per genome per replication

Range	Table - link
Organism	RNA viruses
Reference	Drake JW. Rates of spontaneous mutation among RNA viruses. Proc Natl Acad Sci U S A. 1993 May 1 90(9):4171-5 p.4173 table 1PubMed ID8387212
Method	Mutation rates:Qß-"The A?G mutation rate at an extracistronic site was 3.5×10^-4, taking selection into account (refs 8, 9)." . Poliovirus-"A?C was measured at one site in the replicase gene (ref 11)." VSV-"G?A frequencies were measured at two sites under conditions where phenotypic mixing and selection were negligible (ref 16)." flu A-"an average of 849 bases was sequenced in each of 108 clonal copies of a gene encoding a nonstructural protein (ref 19)." SNV-"A 288-base lacZa target inserted into SNV was used to sequence selectively neutral mutations arising during the three mutable replications of one provirus-to-provirus cycle with negligible selection (ref 20)." RSV-"Mutations were measured in a 1125 base target in a single provirus-to provirus cycle with denaturing gradient gels (ref 28)."
Comments	"The results of the calculations appear in Table 1 Different µg values for a given virus vary widely, the extreme being from 0.1 to 17 for poliovirus. This result is expected from the frequent use of very small mutation targets, often as small as a single transition at a single base: in DNA-based microbes, the mutability of individual sites can vary by several orders of magnitude (refs 29, 30). For poliovirus, the smallest value is based upon a single transversion pathway, and transversions may occur less frequently than transitions (ref 31): on the other hand, the largest value is clearly incompatible with viability." See notes beneath table
Entered by	Uri M
ID	106762