Cytoplasmic volume of HeLa cell

Value 940 µm^3 Range: Table - link µm^3
Organism Human Homo sapiens
Reference Fujioka A, Terai K, Itoh RE, Aoki K, Nakamura T, Kuroda S, Nishida E, Matsuda M. Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes. J Biol Chem. 2006 Mar 31 281(13):8917-26 p.8919 left column top paragraph and supplementary table 1PubMed ID16418172
Method P.8918 right column bottom paragraph: "HeLa and COS-7 cells expressing RFP fused to a nuclear localization signal were trypsinized, suspended in phosphate-buffered saline, and observed under an inverted microscope. Thirty cells for each were imaged for differential interference contrast and fluorescence to measure the diameters of cells and nuclei, respectively. The volumes of cells and nuclei were calculated assuming that they were spheres."
Comments P.8919 left column top paragraph: "The nuclear and cytoplasmic volumes of HeLa cells were 0.22 and 0.94 pl, respectively. Those of COS-7 were 0.14 and 2.4 pl, respectively." See BNID 103719
Entered by Uri M
ID 106664