Value |
5e+7
M^-1×sec^-1
|
Organism |
Bacteria Escherichia coli |
Reference |
Gerstle JT, Fried MG. Measurement of binding kinetics using the gel electrophoresis mobility shift assay. Electrophoresis. 1993 Aug14(8):725-31. p.730 left column 2nd paragraphPubMed ID8404816
|
Primary Source |
Fried MG, Crothers DM. Kinetics and mechanism in the reaction of gene regulatory proteins with DNA. J Mol Biol. 1984 Jan 25 172(3):263-82.PubMed ID6319716
|
Method |
Gel electrophoresis mobility shift assay (emsa) |
Comments |
At 21°C without salt. According to abstract of primary source 5±2.5×10^7M-1s-1 |
Entered by |
Uri M |
ID |
106396 |