Association rate constant (Kon) of lac repressor

Value 2.8e+7 Sec^-1×M^-1 Range: Table - link Sec^-1×M^-1
Organism Bacteria Escherichia coli
Reference Nelson HC, Sauer RT. Lambda repressor mutations that increase the affinity and specificity of operator binding.Cell. 1985 Sep42(2):549-58. p.552 table 3PubMed ID3161621
Method Researchers describe ? repressor mutants that bind operator DNA more tightly, and in some cases more specifically, than wild-type repressor. The amino acid substitutions responsible for these phenotypes were obtained by selecting for intragenic, second-site mutations that restored activity to a mutant repressor. These second-site changes were then separated from the primary mutation, and the DNA binding properties of repressors bearing only the revertant changes were studied.
Comments lac repressor at 22°C, 200 mM KCl, reasonably physiological except perhaps for the 5% DMSO. Value arrived at by dividing Koff (0.022sec^-1, BNID 106393) by equilibrium dissociation constant (7.9×10^-10M, BNID 106391). See note beneath table
Entered by Uri M
ID 106392