Range |
Table - link
|
Organism |
Bacteria Escherichia coli |
Reference |
Kubitschek HE. Increase in cell mass during the division cycle of Escherichia coli B/rA. J Bacteriol. 1986 Nov168(2):613-8. p.617 table 3PubMed ID3536854
|
Method |
A modified Coulter counter
multichannel analyzer system.
In exponentially growing cultures,
the doubling time (T) was calculated for each experiment
from regressions fitted to the logarithm of the observed
values for increasing turbidity, mean cell volume (V) was
determined from observed cell volume distributions, and
mean cell mass (M) was determined as the product of the
mean cell volume and the observed mean buoyant density
(?). In synchronous cultures, doubling times were determined
from increases in both mean cell volume and cell number. |
Comments |
MSGT medium=(M9
salts, 20 g of sucrose and 4 g of glucose per liter, and 0.01%
Hoagland trace element salt solution [1] without selenium). MSGT-CAA medium=(MSGT medium with Casamino
Acids [1 g/liter] added). E. coli B/rA (ATCC 12407). Range of volumes of 1.0-1.6µm^3 given in table. |
Entered by |
Uri M |
ID |
106304 |