| Value |
7.45
Unitless
|
| Organism |
Human Homo sapiens |
| Reference |
Llopis J, McCaffery JM, Miyawaki A, Farquhar MG, Tsien RY. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc Natl Acad Sci U S A. 1998 Jun 9 95(12):6803-8. p.6806 left column bottom line and p.6807 fig. 5bPubMed ID9618493
|
| Method |
Compartment-
Specific Expression of GT-EYFP in Mammalian Cells. Fluorescence microscopy in living intact cell |
| Comments |
"In cells expressing GT-EYFP, the protonophore CCCP (0.15mM) raised pHG to 7.1 (Fig. 5b), even in the presence of active H+ pumping. Bafilomycin A1 added next alkalinized the Golgi only slightly. In the experiment of Fig. 5b, one cell in the field was chosen as negative control because it showed a diffuse fluorescence staining (five times dimmer than that of Golgi area in neighboring cells). This staining likely corresponds to endoplasmic reticulum, because all GT-EYFP is synthesized there before export to the Golgi. This compartment had a resting pH of about 7.45 and, in contrast with pHG, it acidified slightly on CCCP addition (Fig. 5b)." |
| Entered by |
Uri M |
| ID |
105942 |