| Value |
7.91
Unitless
Range: ±0.16 Unitless
|
| Organism |
Mammalian tissue culture cell |
| Reference |
Llopis J, McCaffery JM, Miyawaki A, Farquhar MG, Tsien RY. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc Natl Acad Sci U S A. 1998 Jun 9 95(12):6803-8. p.6805 right column 2nd paragraph and fig.3dPubMed ID9618493
|
| Method |
Compartment-
Specific Expression of EYFPmito in Mammalian Cells. Fluorescence microscopy in living intact cell |
| Comments |
"The normal beating of cardiomyocytes expressing
EYFP-mito suggests the noninvasive nature of these indicators." "The estimated resting pHm was 7.98±0.07 in HeLa cells (n=17 cells from six experiments) and 7.91±0.16 in cardiomyocytes (n=13 cells from six experiments). Similar pH values were obtained in a HeLa cell line stably expressing EYFP-mito." See BNID 105939 |
| Entered by |
Uri M |
| ID |
105940 |