pH of resting mitochondria in living HeLa cell
| Value | 7.98 Unitless Range: ±0.07 Unitless |
|---|---|
| Organism | Human Homo sapiens |
| Reference | Llopis J, McCaffery JM, Miyawaki A, Farquhar MG, Tsien RY. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc Natl Acad Sci U S A. 1998 Jun 9 95(12):6803-8. p.6805 right column 2nd paragraph and fig.3dPubMed ID9618493 |
| Method | Compartment-Specific Expression of EYFPmito in Mammalian Cells. Fluorescence microscopy in living intact cell |
| Comments | "The estimated resting pHm was 7.98±0.07 in HeLa cells (n=17 cells from six experiments) and 7.91±0.16 in cardiomyocytes (n=13 cells from six experiments). Similar pH values were obtained in a HeLa cell line stably expressing EYFP-mito." See BNID 105940 |
| Entered by | Uri M |
| ID | 105939 |