Dry weight composition of autotrophically, mixotrophically and heterotrophically grown cells

Range Table - link Fraction DW
Organism Green algae Chlamydomonas reinhardtii
Reference Boyle NR, Morgan JA. Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii. BMC Syst Biol. 2009 Jan 7 3: 4. p.6 of free online article - table 2PubMed ID19128495
Method Lipids were measured using the chloroform-methanol extraction method of [(ref 45) Ishida et al., 1997]. The amino acid composition was estimated from Gas chromatography-mass spectrometry (GC-MS) analysis of hydrolyzed protein. Chlorophyll a and b were measured [Harris 1989] and subtracted from the total lipid measurement. DNA and RNA were assumed to be constant for all growth conditions the DNA content was determined by [Chiang and Sueoka 1967 PMID 5231756] to be 1.23×10^-7µg per cell and the RNA content was assumed to be 28-fold higher than the DNA content [Valle et al., 1981 PMID 6166650].
Comments For conditions of growth and doubling times see BNID 105914
Entered by Uri M
ID 105924