Range |
Table - link Fraction DW
|
Organism |
Green algae Chlamydomonas reinhardtii |
Reference |
Boyle NR, Morgan JA. Flux balance analysis of primary metabolism in Chlamydomonas reinhardtii. BMC Syst Biol. 2009 Jan 7 3: 4. p.6 of free online article - table 2PubMed ID19128495
|
Method |
Lipids were measured
using the chloroform-methanol extraction method of [(ref 45) Ishida et al., 1997]. The amino acid composition was estimated from Gas
chromatography-mass spectrometry (GC-MS) analysis of
hydrolyzed protein. Chlorophyll a and
b were measured [Harris 1989] and subtracted from the total lipid
measurement. DNA and RNA were assumed to be constant
for all growth conditions the DNA content was
determined by [Chiang and Sueoka 1967 PMID 5231756] to be 1.23×10^-7µg per
cell and the RNA content was assumed to be 28-fold
higher than the DNA content [Valle et al., 1981 PMID 6166650]. |
Comments |
For conditions of growth and doubling times see BNID 105914 |
Entered by |
Uri M |
ID |
105924 |