| Value |
220
mg/ml
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Cayley S, Record MT Jr. Roles of cytoplasmic osmolytes, water, and crowding in the response of Escherichia coli to osmotic stress: biophysical basis of osmoprotection by glycine betaine. Biochemistry. 2003 Nov 4 42(43):12596-609. p.12604 right column bottom paragraph and p.12605 fig.7APubMed ID14580206
|
| Method |
The amount
of RNA in the supernatant was quantified by the orcinol
assay (Herbert et al., 1971 Methods
Microbiol. 513, 209-234.) using total yeast RNA as a standard. The total amount of protein in cells was determined by
the method of Lowry et al. (1951, PMID 14907713) using BSA as a standard. |
| Comments |
At cytoplasmic water amount of 110 µmol/mg of DW [characteristic of cells grown in MBM (and MBM+GB)
at the optimal growth osmolality of ~0.3 Osm]. MBM=MOPS-buffered minimal glucose
medium. GB=glycine betaine |
| Entered by |
Uri M |
| ID |
105904 |