Concentration of protein in cell

Range 200 to 320 mg/ml
Organism Bacteria Escherichia coli
Reference Elowitz MB, Surette MG, Wolf PE, Stock JB, Leibler S. Protein mobility in the cytoplasm of Escherichia coli. J Bacteriol. 1999 Jan181(1):197-203. p.197 left column 2nd paragraphPubMed ID9864330
Primary Source Cayley S, Lewis BA, Guttman HJ, Record MT Jr. Characterization of the cytoplasm of Escherichia coli K-12 as a function of external osmolarity. Implications for protein-DNA interactions in vivo. J Mol Biol. 1991 Nov 20 222(2):281-300 AND Zimmerman SB, Trach SO. Estimation of macromolecule concentrations and excluded volume effects for the cytoplasm of Escherichia coli. J Mol Biol. 1991 Dec 5 222(3):599-620.PubMed ID1960728, 1748995
Comments "The interior of a bacterial cell is an environment crowded with a heterogeneous collection of macromolecules. In E. coli, the concentrations of protein, RNA, and DNA are 200 to 320 mg/ml, 75 to 120 mg/ml, and 11 to 18 mg/ml, respectively (primary sources)." Primary source Cayley et al. 1991 abstract p.281 bottom paragraph:”Since ?cyto decreases with external osmolarity, the concentration of cytoplasmic protein increases from approximately 200 mg/ml in cells grown at 0.10 osm to approximately 320 mg/ml at 1.02 osm.”
Entered by Uri M
ID 108263