Optical density (OD) at 450nm per 10^9cells/ml

Range 0.9 at growth rate of 0.31 hour^-1 to 4.1 at growth rate of 2.4 hour^-1 Unitless
Organism Bacteria Salmonella typhimurium
Reference Aldea M, Herrero E, Esteve MI, Guerrero R. Surface density of major outer membrane proteins in Salmonella typhimurium in different growth conditions. J Gen Microbiol. 1980 Oct120(2):355-67. p.358 table 1 - link PubMed ID6262433
Method P.357 top paragraph: "Growth kinetics were monitored by measuring the absorbance of the culture (at 550 nm for cultures in LB medium and at 450 nm for minimal medium), using a Beckman spectrophotometer, model DU-2 (1 cm light path). The total number of bacteria was determined with a Petroff-Hauser counter, from samples fixed in 0.2% (v/v) formaldehyde at 0°C." For conditions of growth see note above table
Comments Growth rate of 0.25 hours^-1 means doubling time of 2.8hours. Growth rate of 2.4 hours^-1 means doubling time of 17.3min. Organism is S. typhimurium LT2.
Entered by Uri M
ID 105886