Concentration of molybdenum in cells grown aerobically in medium with sodium-molybdate

Range 5.6 Table - link µM
Organism Bacteria Escherichia coli
Reference Scott D, Amy NK. Molybdenum accumulation in chlD mutants of Escherichia coli. J Bacteriol. 1989 Mar171(3):1284-7. free online article p. 1285 table 2PubMed ID2646274
Primary Source 15) Neidhardt, F. C. 1987. Chemical composition of Escherichia coli, p. 3-6. In F. C. Neidhardt, J. I. Ingraham, K. B. Low, B. Magasanik, M. Schaecter, and H. E. Umbarger (ed.), Escherichia coli and Salmonella typhimurium: cellular and molecular biology, vol. 1. American Society for Microbiology, Washington, D.C. AND (18) Singleton, P., and D. Sainsbury. 1978. Dictionary of microbiology, p. 145. John Wiley & Sons, Ltd., Chichester, England.
Method Cells were grown in low-phosphate minimal medium (21) containing 10µg of thiamine per ml, 20 µg of L-histidine per ml, and 0.2% glucose. Where indicated, 20 mM sodium nitrate or 0.1 mM sodium molybdate was included. Mo was measured by electrothermal atomic absorption spectrometry. Molarity of molybdenum in whole cells was calculated by estimating cell volume as 1.6e-15 liter (ref 18) and dry weight of one cell as 2.8e-13 g (ref 15 BNID 103904).
Comments For Mo values under varying conditions see 2nd column in table. Strain SA302 is the one closest to the wild type. See BNID 100136
Entered by Uri M
ID 105672