Molybdenum accumulation in chlD mutants of Escherichia coli

J Bacteriol. 1989 Mar;171(3):1284-7. doi: 10.1128/jb.171.3.1284-1287.1989.

Abstract

The content of molybdenum in wild-type and chlD cells was measured under a variety of growth conditions to determine if cells with a defective chlD gene were able to accumulate molybdenum. The chlD cells accumulated less molybdenum than wild-type cells did but concentrated molybdenum to a level at least 20-fold higher than the concentration in the culture medium. Molybdenum was present within spheroplasts of chlD cells and was not dialyzable. The chlD cells accumulated as much molybdenum as wild-type cells did when grown in medium containing 0.1 mM molybdate; thus, the capability of incorporation of molybdenum into cellular component(s) was equivalent to that of the wild type under these conditions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cytochromes / genetics*
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Genes*
  • Genes, Bacterial*
  • Molybdenum / metabolism*
  • Mutation*
  • Nitrate Reductases / genetics
  • Species Specificity
  • Spheroplasts / metabolism

Substances

  • Cytochromes
  • Molybdenum
  • Nitrate Reductases