Genome size of (large) chromosome
Value | 1.66e+6 bp |
---|---|
Organism | Archaea Methanocaldococcus jannaschii |
Reference | Bult et al., Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii. Science. 1996 Aug 23 273(5278):1058-73. p.1058 right-most column bottom paragraph.PubMed ID8688087 |
Method | p.1058 right column 2nd paragraph:"A whole-genome random sequencing method (Fleischmann et al., 1995 PMID 7542800 and Fraser et al., 1995 PMID 7569993) was used to obtain the complete genome sequence for M. jannaschii A small insert plasmid library [average insert size, 2.5 kilobasepairs(kbp)] and a large-insert ? library (average insert size, 16 kbp) were used as substrates for sequencing. The ? library was used to form a genome scaffold and to verify the orientation and integrity of the contigs formed from the assembly of sequences from the plasmid library." |
Comments | "The M. jannaschii genome consists of three physically distinct elements: (i) a large circular chromosome of 1,664,976 base pairs (bp) (Fig. 1), which contains 1682 predicted protein-coding regions and has a G+C content of 31.4% (ii) a large circular extrachromosomal element (ECE) (H. Zhao et al., Arch. Microbiol. 150,178 (1988)) of 58,407 bp, which contains 44 predicted protein-coding regions and has a G+C content of 28.2% (Fig. 2) and (iii) a small circular ECE (H. Zhao et al., Arch. Microbiol. 150,178 (1988)) of 16,550 bp, which contains 12 predicted protein-coding regions and has a G+C content of 28.8% (Fig.2)" Total genome size=1,739,933bp. Total gene number=1,682+44+12=1,738genes |
Entered by | Uri M |
ID | 105501 |