Genome size of chromosome of strain S0385 (livestock-associated MRSA)
| Value | 2.87e+6 bp Range: Table - link bp |
|---|---|
| Organism | Bacteria Staphylococcus aureus |
| Reference | Schijffelen MJ, Boel CH, van Strijp JA, Fluit AC. Whole genome analysis of a livestock-associated methicillin-resistant Staphylococcus aureus ST398 isolate from a case of human endocarditis. BMC Genomics. 2010 Jun 14 11: 376. p.2 left column bottom paragraph & table 1PubMed ID20546576 |
| Method | "DNA from isolate S0385 was sequenced using pyrophosphate sequencing technology to 16 fold coverage by the 454 group of Roche Applied Sciences. Resulting reads were assembled into contigs and were placed into the correct order and direction using the USA300 FR3757 genome as a scaffold. All gaps between contigs were closed by conventional PCR using primers based on the sequence of flanking contigs followed by sequencing of the products." |
| Comments | "The MRSA [methicillin-resistant Staphylococcus aureus] ST398 genome consists of a circular chromosome of 2,872,582 bp, as well as 3 circular plasmids [sizes of plasmids 1-3 are 5,246bp, 4,381bp and 3,158bp, respectively]. The size of the chromosome is comparable to that of other sequenced S. aureus strains. [Researchers] identified 2,699 ORFs, 6 ribosomal RNA operons, and 24 mobile genetic elements, including 12 insertion sequence (IS) elements. The general features of the genome are summarized in Table 1." |
| Entered by | Uri M |
| ID | 105499 |