Generation time of H. influenzae type b in rat blood in vitro
| Value | 23.6 min Range: ±0.9 min |
|---|---|
| Organism | Bacteria Haemophilus influenzae |
| Reference | Rubin LG. Comparison of in vivo and in vitro multiplication rates of Haemophilus influenzae type b. Infect Immun. 1986 Jun52(3):911-3. p. 912 table 1 Table - link PubMed ID3486830 |
| Method | A streptomycin-resistant mutant of H. influenzae type b strain Rd/b+, a laboratory transformant, was used because it is highly virulent in rats. Bacteria were grown to mid-logarithmic phase in supplemented brain heart infusion broth and inoculated in vitro into rat blood and broth, and in vivo into rats that had undergone splenectomy and a sham operation. See comments under table for more details. |
| Entered by | Uri M |
| ID | 105461 |