| Value |
2E-06
events/cells plated
|
| Organism |
Budding yeast Saccharomyces cerevisiae |
| Reference |
Dong C, Poulter RT, Han JS. LINE-like retrotransposition in Saccharomyces cerevisiae. Genetics. 2009 Jan181(1):301-11PubMed ID18957700
|
| Method |
scZorro3 is controlled by the GAL1 inducible promoter, and an antisense reporter (mHIS3AI) interrupted with an intron on the scZorro3 sense strand is placed in the 3'-UTR. Only after scZorro3 transcription, splicing, and reverse trascription/integration does the marker produce functional HIS3 protein (fig 1b). When placed on a high-copy plasmid and introduced into S. cerevisiae, this led to scZorro3-dependent HIS+ colony formation (Figure 1C), indicating that retrotransposition occurred. To examine scZorro3 retrotransposition from a more “natural” habitat, researchers integrated scZorro3mHIS3AI into chromosome II, along with mutants predicted to abolish the function of activities required for L1 retrotransposition. Under the conditions of their assay, wild-type scZorro3 retrotransposed with a frequency of ~2×10^-6 events/cells plated. |
| Comments |
Zorro3 is a member of the L1 clade of non-LTR retrotransposons from the distantly related Candida albicans that is known to be active for retrotransposition in its host (Goodwin et al. 2007, PMID: 17683538) |
| Entered by |
Uri M |
| ID |
105450 |