| Value |
4
years
|
| Organism |
Generic |
| Reference |
Wolfenden R, Snider MJ. The depth of chemical time and the power of enzymes as catalysts. Acc Chem Res. 2001 Dec34(12):938-45. p.940 table 1 Table - link PubMed ID11747411
|
| Primary Source |
Thompson, J. E. Kutateladze, R. G. Schuster, M. C. Venegas, R. Messmore, J. M. Raines, R. T. Limits to Catalysis by Ribonuclease A. Bioorg. Chem. 1995, 23, 471-481. |
| Method |
Reaction rates were measured at series of elevated temperatures,
following them to completion. The resulting Arrhenius
plot, if linear, can then be extrapolated to 25 °C. In much of this work, reaction mixtures were sealed in
quartz, heated over various intervals, and then opened for
analysis by proton NMR. The vapor pressure of water does
not change enough to affect reaction rates significantly,
but aqueous samples tend to burst at temperatures above
250 °C (40 atm). Explosions are avoided by placing
reaction tubes in water inside a steel bomb, equalizing
pressure across the wall of each tube. PTFE vessels must
be used for alkaline solutions which attack quartz. |
| Comments |
The phosphodiester linkages
of RNA, destabilized by the neighboring 2'-hydroxyl group,
are cleaved with an estimated half-time of 4 years. Halftime at 100°C is 9 days. See table link. |
| Entered by |
Uri M |
| ID |
105354 |