Halftime of hydrolysis event of RNase A (123 peptide bonds) at 25°C

Value 4 years
Organism Generic
Reference Wolfenden R, Snider MJ. The depth of chemical time and the power of enzymes as catalysts. Acc Chem Res. 2001 Dec34(12):938-45. p.940 table 1 Table - link PubMed ID11747411
Primary Source Radzicka, A. Wolfenden, R. Rates of Uncatalyzed Peptide Bond Hydrolysis in Neutral Solution and the Transition State Affinities of Proteases. J. Am. Chem. Soc. 1996, 118, 6105-6109.
Method Carboxypeptidase, endopeptidase and dipeptide cleavage reactions were modeled by a suitable derivative of glycylglycine, and could be followed by proton NMR in the temperature range near 150 °C. In each case, the linear Arrhenius plot yielded a half-time of approximately 400 years at 25 °C (primary source).
Comments Value calculated by dividing halftime of spontaneous protein hydrolysis at 25°C, 400 years (BNID 105352) by number of amino acids in RNase A. Halftime of hydrolysis event of peptide bond in RNase A at 100°C is 7 hours. See table link
Entered by Uri M
ID 105356