| Reference |
Fujioka A, Terai K, Itoh RE, Aoki K, Nakamura T, Kuroda S, Nishida E, Matsuda M: Dynamics of the Ras/ERK MAPK cascade as monitored by fluorescent probes. J Biol Chem 2006, 281:8917-8926. Supplementary table 1PubMed ID16418172
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| Method |
Morphometric Analysis—HeLa and COS-7 cells expressing RFP fused to a nuclear localization signal were trypsinized, suspended in phosphate-buffered saline, and observed under an inverted microscope. Thirty cells for each were imaged for differential interference contrast and fluorescence to measure the diameters of cells and nuclei, respectively. The volumes of cells and nuclei were calculated assuming that they were spheres. The nuclear and cytoplasmic volumes of HeLa cells were 0.22 and 0.94 pl, respectively. Those of COS-7 were 0.14 and 2.4 pl, respectively. |