| Value |
18
Sec^-1
|
| Organism |
Bacteria Escherichia coli |
| Reference |
Chen JF, Guo NN, Li T, Wang ED, Wang YL. CP1 domain in Escherichia coli leucyl-tRNA synthetase is crucial for its editing function. Biochemistry. 2000 Jun 6 39(22):6726-31. Table - link PubMed ID10828991
|
| Method |
Construction of a Plasmid Containing the Gene Encoding
His6-Tagged LeuRS-B. Cloning and Expression of the Gene Encoding CP1Leu. Assay of Enzyme ActiVity. Circular Dichroism (CD) Spectroscopy. |
| Comments |
ATP-PPi exchange kinetics of enzymes were determined in the
presence of 0.02-0.2 mM leucine and 5-50 mM methionine or
isoleucine. All the data in this table were the average values with a
variation of <10% from three independent determinations. The
discrimination factors were calculated from the equation DMet or Ile )
[(kcat/Km)Leu]/[(kcat/Km)Met or Ile]. For value of 0.07 sec^-1 in human mitochondrial LeuRS see BNID 104979 |
| Entered by |
Uri M |
| ID |
104981 |