DNA synthesis rate in AMPD2 amplified gene region

Value 1832 bp/min
Organism Chinese hamster ovary (CHO)
Reference Anglana M, Apiou F, Bensimon A, Debatisse M. Dynamics of DNA replication in mammalian somatic cells: nucleotide pool modulates origin choice and interorigin spacing. Cell. 2003 Aug 8 114 (3): 385-94. p.389 right column 2nd paragraphPubMed ID12914702
Method "[Researchers] have a longstanding interest in the study of a specific chromosome subregion, amplified in Chinese hamster cells selected for resistance to coformycin, an inhibitor of adenylate deaminase2 (AMPD2)." "DNA from asynchronous cells was sequentially labeled with IdU and CldU." In DNA (chromosome) combing the DNA is stretched so that hybridization with probes is possible. By periodically adding radioactive label of different colors origins of replication and fork replication rate can be determined.
Comments "Focusing on the AMPD2 locus, [researchers] analyzed 37 fibers that were labeled by FISH and contained replication signals (Supplemental Figure S1 at link Like in the whole genome, the average fork speed increased in the AMPD2 region (mean rate: 1832 bp/min)." As previously observed, in this study too the fork movement rate was variable within each cell line.
Entered by Uri M
ID 104937