Base-ratios of E. coli ML30 RNA made under varying conditions of growth
Range | Table - link |
---|---|
Organism | Bacteria Escherichia coli |
Reference | Nierlich DP. Regulation of ribonucleic acid synthesis in growing bacterial cells. II. Control over the composition of the newly made RNA. J Mol Biol. 1972 Dec 30 72(3):765-77PubMed ID4573848 |
Method | Portions (5 ml.) of culture received H3 (32)P04, the quantities varying from O.5 mCi for control and shift-up cultures to 4.0 mCi for shift-down cultures. After 2 min the cells were collected over ice and centrifuged for 6 min at 30,000 g. The count rates were corrected for the decay of 32P during the counting, and where count rates exceeded 100,000 cts/min, corrections for coincidence losses were made. See table for more measurement details |
Comments | Table gives base ratio of RNA and percent of stable RNA out of total RNA under different growth conditions |
Entered by | Uri M |
ID | 104885 |