| Value |
15
Hours
|
| Organism |
Teleost fish Apteronotus leptorhynchus |
| Reference |
Sîrbulescu RF, Ilies I, Zupanc GK. Structural and functional regeneration after spinal cord injury in the weakly electric teleost fish, Apteronotus leptorhynchus. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2009 Jul195(7):699-714PubMed ID19430939
|
| Method |
To determine the spatiotemporal pattern of apoptosis after
SCI, researchers used active caspase-3, an executioner caspase, as a
marker of apoptosis. Transection lesions of the spinal cord
were applied by amputating 1-cm caudal segments of the
tail, and were followed by post-amputation survival times of
0–50 days. A highly significant change in the number of
apoptotic cells was observed [F(12,26) = 7.126, P < 0.001,
one-way ANOVA]. |
| Comments |
An increase in the number of caspase-3-
positive cells, relative to baseline levels, was observed around 12 h of survival, reaching maximum levels after 15 h
(P < 0.05, Duncan post-hoc test Fig. 3a–f, h). After 24 h
post-lesion, the apoptotic cells declined in number, first
sharply until 5 days of survival, then more slowly until baseline
levels were reached around 30 days post amputation
(Fig. 3 h). Teleost fish and urodele amphibians (salammanders) differ markedly from mammals in their much higher ability to recover from spinal chord and brain injury. |
| Entered by |
Uri M |
| ID |
104869 |